Stop Solutions for TMB

Our Stop Solutions are acid-based solutions for use with our TMB ELISA substrates. TMB reacts with peroxide in the presence of horseradish peroxidase to produce a soluble blue product. This reaction can be stopped by the addition of an equal amount of stop solution, resulting in a soluble yellow product that can be read at 450nm.

Supplied in liquid format. For general use to stop colour development in ELISA.
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Supplied in liquid format. This solution can prevent the formation of precipitate of black TMB end product in ELISA.

This stopping solution is capable of retaining the OD reading for over 2 hours after stopping.