Two-component TMB solution for ELISA

This type of TMB solution is supplied in two parts. Part A is a H2O2 -containing solution and Part B a TMB-containing one, in separate bottles. Mix them at a 1:1 ratio just before use.

Like the one-component TMB solutions, this version of TMB solution is also designed for ELISA procedures. The mixed TMB solution of parts A & B, when reacted with HRP, produces a blue, soluble end product which can be read at 655 nm. The reaction can be stopped using acid solution (e.g. 0.5 M H2SO4, 0.5 M HCl, 1 M H3PO4) to convert the blue radical to a yellow diimine which can be read at 450 nm. This has been reported to increase the sensitivity of the assay.

  • Post termination stability: no change in OD within one hour, and <10% drop of OD within 24 hours after the addition of stopping solution.

Stable for at least 12 months at room temperature, or 36 months at + 2-8 °C.